Organism: Staphylococcus aureus

Target: NMN phosphatase; Class B acid phosphatase precursor

EC:3.1.3.2 :Acid phosphatase

http://www.brenda-enzymes.org/literature/lit.php4?e=3.1.3.2&r=663565

Background and Disease: S. Aureus has a role in causing food poisoning, atopic dermatitis, staphylococcal scalded skin syndrome, or Ritter's disease in neonates.

Enzyme page on Brenda:
http://www.brenda-enzymes.org/php/result_flat.php4?ecno=3.1.3.2


Reaction pathway:

http://www.brenda-enzymes.org/php/result_flat.php4?ecno=3.1.3.2&Suchword=t.+cruzi&organism[]=Staphylococcus+aureus&show_tm=0


enzyme assay info: http://www.sigmaaldrich.com/technical-documents/protocols/biology/enzymatic-assay-of-acid-phosphatase.html

REAGENTS:

Sodium citrate tribasic dehydrate: 174.00

Sodium hydroxide solution: 199.00

Gene #:12865799

Essentiality of protein: None

Inhibitors: Vancomycin

Image of protein:



Coding DNA Sequence link:
http://www.ncbi.nlm.nih.gov/nuccore/JH806568.1

Amino Acid sequence:
1 mraaplllar aaslalascf cffcwldrsv lakelkfvtl vfrhgdrspi dtfptdpike
61 sswpggfggl tglgmeqhye lgeyirkryr kflndsykhe qvyirstdvd rtlmsrmtnl
121 aalfppegvs iwnpillwqp ipvhtvplse dqllylpfrn cprfqelese tlkseefqkr
181 lhpykdfiat lgklsglhgq dlfgiwskvy dplysesvhn ftlpswated tmtklrelse
241 lsllslygih kqkeksrlqg gvlvneilnh mkratqipsy kklimysahd ttvtglqmal
361 gpvipqdwst evmttnshqg tedstd


Primer Design:

1 ATGCGTGCAGCACCTCTGCTCCTGGCCCGTGCGGCCTCTCTGGCGCTGGCATCTTGC 57
2 TCTTTCGCCAGAACAGAGCGGTCCAGCCAACAGAAGAAGCAGAAGCAAGATGCCAGCGCC 60
3 GCTCTGTTCTGGCGAAAGAACTGAAGTTCGTTACTCTGGTTTTCCGTCACGGTGACCGTT 60
4 GAAGATTCTTTAATCGGATCGGTCGGAAAGGTATCGATCGGAGAACGGTCACCGTGACGG 60
5 GACCGATCCGATTAAAGAATCTTCTTGGCCGCAGGGCTTTGGTCAACTGACGCAGCTGGG 60
6 GCGCTTACGGATGTACTCGCCCAGTTCGTAGTGCTGTTCCATACCCAGCTGCGTCAGTTG 60
7 CGAGTACATCCGTAAGCGCTACCGTAAATTCCTGAACGACTCCTACAAGCATGAACAGGT 60
8 GACATGAGGGTACGGTCAACGTCAGTAGAACGAATGTAAACCTGTTCATGCTTGTAGGAG 60
9 GTTGACCGTACCCTCATGTCTCGTATGACGAACCTGGCGGCACTCTTCCCGCCTGAGGGC 60
10 ACCGGGATCGGCTGCCACAGCAGGATTGGATTCCAGATAGAGACGCCCTCAGGCGGGAAG 60
11 GCAGCCGATCCCGGTTCACACCGTTCCACTCTCTGAAGATCAACTCCTGTACCTGCCGTT 60
12 AGGGTTTCAGATTCGAGTTCCTGGAAACGCGGGCAGTTGCGGAACGGCAGGTACAGGAGT 60
13 GGAACTCGAATCTGAAACCCTGAAATCTGAGGAGTTCCAGAAACGTCTCCACCCGTACAA 60
14 CCGTGCAGGCCAGACAGTTTGCCCAGGGTCGCGATGAAGTCTTTGTACGGGTGGAGACGT 60
15 TGTCTGGCCTGCACGGTCAGGATCTGTTTGGTATCTGGTCTAAAGTTTACGACCCGCTGT 60
16 CCCAAGATGGCAGAGTGAAGTTGTGAACAGACTCGGAGTACAGCGGGTCGTAAACTTTAG 60
17 CTTCACTCTGCCATCTTGGGCGACTGAAGACACCATGACTAAACTGCGTGAGCTGTCTGA 60
18 TCTTTTTGTTTGTGGATACCGTACAGAGACAGGAGGCTCAGTTCAGACAGCTCACGCAGT 60
19 GTACGGTATCCACAAACAAAAAGAAAAATCTCGTCTGCAAGGTGGCGTTCTCGTTAATGA 60
20 GCTCGGGATCTGGGTCGCACGTTTCATGTGGTTGAGAATTTCATTAACGAGAACGCCACC 60
21 CGACCCAGATCCCGAGCTACAAAAAACTGATCATGTATTCTGCGCATGACACGACCGTTA 60
22 GCAGCAGGCCGTTGTAAACGTCCAGGGCCATCTGGAGGCCGGTAACGGTCGTGTCATGCG 60
23 TTACAACGGCCTGCTGCCGCCTTACGCCTCTTGTCACCTCACCGAACTGTACTTCGAAAA 60
24 TCTCGTTGCGATAGTACATTTCAACGAAATATTCACCTTTTTCGAAGTACAGTTCGGTGA 60
25 TGAAATGTACTATCGCAACGAGACCCAGCACGAACCGTATCCGCTGATGCTCCCAGGTTG 60
26 CCAACGAGCTCCGCGAAACGTTCCAGCGGGCAAGACGGAGAGCAACCTGGGAGCATCAGC 60
27 TCGCGGAGCTCGTTGGTCCGGTTATCCCGCAGGACTGGTCCACCGAAGTTATGACCACTA 60
28 GTCGGTAGAGTCCTCGGTGCCCTGGTGAGAGTTAGTGGTCATAACTTCGGTGG 53

Link to GDocs Output:

https://docs.google.com/file/d/0B4O2KqKh2q_-WER0Q3FuOVl6RkU/edit